FIG. 6.

Use of HMA to determine HIV relatedness. Heteroduplex mobilities versus DNA distance of sequences of the region of HIV-1 Env from V3 to V5 from the same individuals and from individuals infected with the same (intrasubtype) or different (intersubtype) subtypes of HIV-1. Mobilities were determined by dividing the average distance of migration of the heteroduplex bands by the distance of migration of the homoduplex band. Cloned fragments of the patient proviral DNA were used as starting material. DNA distances (i.e., divergence) were determined by counting mismatches in the best alignment of the sequences, disregarding bases that are unpaired due to insertions or deletions. (Inset) Polyacrylamide (5%) gel stained with ethidium bromide showing representative heteroduplexes from the comparisons discussed above. Courtesy of E. L. Delwart and J. I. Mullins.