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. Author manuscript; available in PMC: 2022 Jul 17.
Published in final edited form as: Nat Metab. 2022 Jan 17;4(1):44–59. doi: 10.1038/s42255-021-00515-3

Extended Data Fig. 5. Tubastatin increases hypothalamic leptin signaling.

Extended Data Fig. 5

Wild-type DIO mice were treated with i.p. vehicle of tubastatin (n=3 mice per group), and co-treated with i.p. leptin. Mice were perfused, and hypothalamic STAT3 phosphorylation was analyzed by immunofluorescent staining. Arcuate nucleus (ARC) (P=0.0054) and dorsomedial hypothalamic (DMH) (P=0.094) confocal images of p-STAT3Y705 stainings (left) and the quantification of the fluorescent intensities (right bar graphs) Scale bar: 20μm. The experiment was conducted in two independent cohorts. *P<0.05, **P<0.01, ***P<0.001 as analyzed by unpaired two-tailed t-test.