Fig. 4.

Characterization of the complex stabilities. (A) Protein–RNA complex stability at different temperatures, pH, and salt conditions. Green: no protein is detected from Western blot; increasing red intensity: increasing amount of protein detected on Western blot of the pull-down flow-through fraction (for uncropped Western blots and fractions remaining bound to the beads, see supplementary fig. S4G, Supplementary Material online). (B) f58rRNA digestion by RNase A in presence or absence of CL11, CL11-M, and CL11-E protein in the buffer R with and without 20 mM EDTA at 30°C.