FIGURE 1.

Behavioural effects produced by Galanin 2 receptor agonist (M1145) and the Neuropeptide Y Y1 receptor (NPY Y1) agonist alone and in combination on retrieval in the Object-in-Place memory task. (A) Schematic representation of the procedures followed to perform the behavioural experiments in the Object-in-Place task. The animals performed the task in three phases, separated 24 h from each other, where they explored freely during 10 min in the habituation phase without objects, 3 min in the training phase with four different objects, and finally 3 min in the test phase with two of the objects with the exchanged position. The pharmacological treatments were administered intranasally to the different groups of animals 24 h before the testing phase. (B) The graphic shows the ability of rats to discriminate the exchanged objects at 24 h post-training after the administration of M1145 in combination with the NPY Y1 agonist in the Object-in-Place task. The response induced by M1145 and the NPYY1 coadministration after the acquisition phase improves object-in-place performance following a 24-h delay. Moreover, this effect is blocked by the GAL 2 receptor (GALR2) antagonist M871. Data represent mean ± SEM of the discrimination ratio during the 3 min of the test phase. N = 6 animals in each group. *p < 0.05 vs. the rest of the groups according to one-way ANOVA (F4, 25 = 3.56) followed by Newman-Keuls post-hoc test. Abbreviations: Control = Distilled water; M1145 = Galanin 2 receptor agonist 132 µg; Y1R = Y1R receptor agonist [Leu³¹-Pro³⁴]NPY 132 µg; M1145 + Y1R = Coadministration of M1145 and Y1R; M1145 + Y1R + M871 = Coadministration of M1145, Y1R and GALR2 antagonist M871 132 µg.