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. 2022 Feb 17;13:838328. doi: 10.3389/fimmu.2022.838328

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Copyright © 2022 Trevelin, Pickering, Todd, Bishop, Pitcher, Garrido Mesa, Montorsi, Spada, Petrov, Green, Shankar-Hari, Neil and Spencer

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of SARS-CoV-2 in tissue samples along the GI tract. (A-C) Evaluation of SARS-CoV-2 presence using RT-qPCR in different FFPET samples from 9 COVID-19 deceased patients and 4 pre-COVID patients used as controls. Dots represent the viral RNA level from each sample and were coloured by patient. The lines represent the median values per organ (B) or patient (C). (D) IHC for SARS-CoV-2 spike and nucleocapsid proteins in an ileal sample from patient 20.8 showing the virus presence in the epithelium (panel on the left) and sub-mucosal macrophages (panels on the right). Scale bar for image on the left: 100μm. Sale bars for images on the right: 20μm. The dashed line delimitates the GALT.