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. 2022 Feb 17;13:838328. doi: 10.3389/fimmu.2022.838328

Figure 2.

Figure 2

Peyer’s patches (PP) from COVID-19 patients lose germinal centre. (A, B) IHC for CD45RB (brown) and CD10 (red) in ileal FFPE samples from COVID-19 patients with different local levels of SARS-CoV-2 viral RNA. Images on the top show the whole sections and ileal follicles are highlighted in each of the bottom images. The images on the left represent a sample from one control; the images in the middle represent a sample from a COVID-19 patient with high local levels of viral RNA; and the images on the right represent a COVID-19 patient with low local levels of viral RNA. (B) CD10:CD45RB area ratio. Data is shown as mean ± SEM. (n = 4 controls and 9 patients). Two tailed Mann-Whitney t test. *P < 0.05. (C) Representative images from histoCAT showing CD3 (green), CD20 (magenta), E-cadherin (orange) and CD68 (white) signals in ileal samples. (D) Representative images from histoCAT showing CD45RB (magenta) and BCL6 (green) signals in PP follicle on the left, and mean BCL6 signals in T and B cells on the right. (E) Percentages of BCL6+PD1+ cells T cells. Data is shown as mean ± SEM. (n = 4 controls and 5 patients). Kruskal-Wallis followed Dunn’s post-test. *P < 0.05, **P < 0.001.