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. 2022 Jan 25;220(3):iyac013. doi: 10.1093/genetics/iyac013

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© The Author(s) 2022. Published by Oxford University Press on behalf of Genetics Society of America.

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Fig. 5. — Additional loci govern response to the mutation. a) We generated BY × 3S crosses in which all segregants carried mrp20-A105E. Two crosses were performed: 1 in which all segregants carried MKT1^BY and 1 in which all segregants carried MKT1^3S. Tetrads were dissected and spores were phenotyped for growth on ethanol. b) Each of the new crosses showed increased growth that extended from inviable to wild type, differing from the more qualitative bimodal phenotypes seen among the original mrp20-A105E MKT1 segregant populations. c) Linkage mapping identified a total of 16 loci that influenced growth. After 4 iterations of a forward regression, no additional loci were identified.