Fig. 7.
Influence of a lack in Atp12 or subunit 6 on the steady state levels and assembly of subunit 9. a) Steady states levels of subunit 9 in atp12Δ and atp6Δ yeasts. Total protein extracts from WT, atp6Δ, and atp12Δ strains grown in rich galactose medium were prepared and separated by SDS–PAGE in a 12% polyacrylamide gel. The proteins were transferred to a nitrocellulose membrane, and probed with antibody against subunit 6 (Atp6), subunit 9 (Atp9), and porin (Por1). The reported values (expressed as %WT) were calculated from 3 independent experiments. Statistical significance of the differences between samples is indicated. b) Assembly of subunit 9 in atp12Δ and atp6Δ yeasts. Mitochondria were isolated from WT, atp6Δ, and atp12Δ strains grown in rich galactose medium. Digitonin extracts containing the same quantity of proteins (50 µg) were prepared and separated in 3–10% polyacrylamide BN gel, transferred to a PVDF membrane, and probed with antibodies against subunit 9. The F1–FO monomers and dimers, and subunit 9-ring are identified in the left-hand margin.