FIGURE 8.

Evaluation of a PAM for the human sweet taste receptor in the DMR and FLIPR assays. (A) R2/R3 U2OS cells were stimulated with increasing concentrations of sucralose in the presence and absence of 25 μM SE-2 and DMR responses were monitored on the Epic^® reader. Depicted are the kinetics corresponding to the effect of SE-2 on 31.25 μM sucralose. (B) Depiction of the full sucralose dose-response analysis of the experiment described in (A). The rectangles include data points from which the kinetics in Panel A were taken. (C) R2/R3 U2OS cells were transduced with Gα₁₅ baculovirus. After 48 hours, cells were loaded with Fluo4 and stimulated with increasing concentrations of sucralose in the presence and absence of 25 μM SE-2 and responses were monitored on the FLIPR. Curves are representative of three independent experiments and data points correspond to an average and standard deviation of a triplicate determination.