TABLE 2.

Transduction coefficients, ΔlogR and ΔΔlogR values obtained from dose-response FLIPR-Gα₁₅ and DMR data fitting using the operational model. Ligand bias was estimated as described in Materials and Methods.

Agonist	ΔlogR FLIPR			ΔlogR DMR			ΔΔlogR				Bias factor
	Mean	Sem	N	Mean	Sem	N	Mean	Sem	Df	95% confidence interval
S819	3.11	0.03	3	2.52	0.04	4	0.59	0.05	5	0.45 to 0.73	3.9
S5227	2.52	0.08	3	1.84	0.08	4	0.68	0.12	5	0.38 to 0.97	4.8
Neotame	1.65	0.03	4	1.15	0.04	3	0.50	0.05	5	0.37 to 0.67	3.2
P-4000	1.67	0.02	3	1.31	0.14	3	0.36	0.14	4	−0.04 to 0.75
S1313	2.14	0.04	4	1.91	0.20	3	0.22	0.20	5	−0.29 to 0.74
S679	2.15	0.03	4	2.07	0.15	3	0.08	0.15	5	−0.31 to 0.47
Superaspartame	1.17	0.03	4	1.04	0.05	4	0.13	0.06	6	−0.01 to 0.27
Alitame	0.52	0.04	4	0.35	0.03	4	0.18	0.05	6	0.05 to 0.31	1.5
SC-45647	1.45	0.02	4	1.38	0.10	6	0.07	0.10	8	−0.17 to 0.31
Dulcin	0.38	0.06	4	0.20	0.05	9	0.18	0.08	11	0.00 to 0.36
Rebaudioside A	0.38	0.05	4	0.22	0.15	3	0.16	0.15	5	−0.24 to 0.56
Stevioside	0.13	0.01	4	0.09	0.12	3	0.04	0.13	5	−0.29 to 0.36
Saccharin	−0.47	0.21	4	−0.31	0.09	3	−0.16	0.23	5	−0.74 to 0.42
Aspartame	−0.74	0.05	4	−0.72	0.08	3	−0.03	0.10	5	−0.27 to 0.22
Acesulfame K	−0.74	0.02	4	−0.99	0.09	3	0.26	0.09	5	0.01 to 0.50	1.8