Figure 2.

Dynamic changes in the sci-ATAC atlas reflect dynamic transcription factor occupancy and identifies both the regulators and the enhancers in each lineage

(A) Heatmap of accessibility deviation scores for TF-bound regions (from bulk ChIP data) per cell population.

(B) UMAPs with cells colored by accessibility deviation score for the indicated TF using bulk ChIP data from specific time points.

(C) Heatmap of accessibility deviation scores by cell population and developmental time (hours) for sites occupied by selected TFs (ChIP data from modERN). Time points with <5% of cells of a given population were excluded.

(D) Heatmaps of average accessibility (Z score) at differentially accessible ATAC-seq peaks at regulatory elements (left) and genes (right) in each cell population.

(E) Embryos showing the expression of luna in the visceral muscle, Nk7.1 and CG14655 in the somatic muscle; in situ hybridization of the gene (green), and a tissue marker (magenta, biniou for visceral muscle, Mef2 for somatic muscle); DAPI-stained nuclei (gray).