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. 2022 Feb 17;10:841017. doi: 10.3389/fcell.2022.841017

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Copyright © 2022 Dietrichs, Grimm, Sahana, Melnik, Corydon, Wehland, Krüger, Vermeesen, Baselet, Baatout, Hybel, Kahlert, Schulz, Infanger and Kopp.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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qPCR expression changes of selected genes and their relationship in STRING protein-protein interaction networks. (A) qPCR gene expression fold changes of the 24 h RPM-exposed PC-3 samples (AD and MCS) in relation to 1g. The red and green colors indicate upregulated and downregulated genes in RPM-exposed samples respectively. Significant regulations are indicated by black asterisks (p < 0.05). (B) qPCR gene expression fold changes of liquid overlay derived MCS cell aggregates in relation to controls. (C,D) Network of the functional interaction of genes and their products (C) analyzed in this study and (D) differtially regulated in liquid overlay derived MCS cell aggregates. The analysis was performed by STRING 11.0 (https://string-db.org/) and combined scores are visualized by Cytoscape 3.8.2. Affiliation to functional gene groups is color-coded. Red asterisks indicate genes known to be involved in prostate cancer gravity sensing.