TABLE 1.
Primers used for qPCR analyses.
| Gene | Primer name | Sequence 5′–3′ |
|---|---|---|
| 18S rRNA | 18s-F | GGAGCCTGCGGCTTAATTT |
| 18s-R | CAACTAAGAACGGCCATGCA | |
| ACTB | ACTB-F | TGCCGACAGGATGCAGAAG |
| ACTB-R | GCCGATCCACACGGAGTACT | |
| AKT1 | AKT1-F | CTTCTATGGCGCTGAGATTGTG |
| AKT1 | CAGCATGAGGTTCTCCAGCTT | |
| CASP3 | CASP3-F | CTCCAACATCGACTGTGAGAAGTT |
| CASP3-R | GCGCCAGCTCCAGCAA | |
| CASP8 | CASP8-F | TGCAAAAGCACGGGAGAAAG |
| CASP8-R | CTCTTCAAAGGTCGTGGTCAAAG | |
| CASP9 | CASP9-F | CTCCAACATCGACTGTGAGAAGTT |
| CASP9-R | GCGCCAGCTCCAGCAA | |
| COL1A1 | COL1A1-F | ACGAAGACATCCCACCAATCAC |
| COL1A1-R | CGTTGTCGCAGACGCAGAT | |
| CXCL8 | CXCL8-F | TGGCAGCCTTCCTGATTTCT |
| CXCL8-R | GGGTGGAAAGGTTTGGAGTATG | |
| EGF | EGF-F | TGCCAGCTGCACAAATACAGA |
| EGF-R | TCTTACGGAATAGTGGTGGTCATC | |
| EGFR | EGFR-F | TTGCCGCAAAGTGTGTAACG |
| EGFR-R | GAGATCGCCACTGATGGAGG | |
| EZR | EZR-F | GCAATCCAGCCAAATACAACTG |
| EZR-R | CCACATAGTGGAGGCCAAAGTAC | |
| FLT1 | FLT1-F | CCCTCGCCGGAAGTTGTAT |
| FLT1-R | GATAATTAACGAGTAGCCACGAGTCAA | |
| FN1 | FN1-F | AGATCTACCTGTACACCTTGAATGACA |
| FN1-R | CATGATACCAGCAAGGAATTGG | |
| HIF1A | HIF1A-F | TGCTTTAACTTTGCTGGCCC |
| HIF1A-R | AGTTTCTGTGTCGTTGCTGC | |
| IL1A | IL1A-F | AGTAGCAACCAACGGGAAGG |
| IL1A-R | AGGCTTGATGATTTCTTCCTCTGA | |
| IL1B | IL1B-F | TTCGAGGCACAAGGCACAA |
| IL1B-R | TGGCTGCTTCAGACACTTGAG | |
| IL6 | IL6-F | CGGGAACGAAAGAGAAGCTCTA |
| IL6-R | GAGCAGCCCCAGGGAGAA | |
| IL7 | IL7-F | CCAGTTGCGGTCATCATGACTA |
| IL7-R | TGATGCTACTGGCAACAGAACA | |
| KDR | KDR-F | TCTTCTGGCTACTTCTTGTCATCATC |
| KDR-R | GATGGACAAGTAGCCTGTCTTCAGT | |
| KRT8 | KRT8-F | GATCTCTGAGTGAACCGGAACA |
| KRT8-R | GCTCGGCATCTGCAATGG | |
| LAMA3 | LAMA3-F | AAAGCAAGAAGTCAGTCCAGC |
| LAMA3-R | TCCCATGAAGACCATCTCGG | |
| MMP9 | MMP9-F | CCTGGAGACCTGAGAACCAATC |
| MMP9-R | TTCGACTCTCCACGCATCTCT | |
| MSN | MSN-F | GAAATTTGTCATCAAGCCCATTG |
| MSN-R | CCATGCACAAGGCCAAGAT | |
| MTOR | MTOR-F | ATCTTGGCCATAGCTAGCCTC |
| MTOR-R | ACAACTGGGTCATTGGAGGG | |
| PIK3CB | PIK3CB-F | AGAAAAGTTTGGCCGGTTCC |
| PIK3CB-R | GCAGTCAACATCAGCGCAAA | |
| RDX | RDX-F | GAAAATGCCGAAACCAATCAA |
| RDX-R | GTATTGGGCTGAATGGCAAATT | |
| SPP1 | SPP1-F | CGAGGTGATAGTGTGGTTTATGGA |
| SPP1-R | CGTCTGTAGCATCAGGGTACTG | |
| TGFB1 | TGFB1-F | CACCCGCGTGCTAATGGT |
| TGFB1-R | AGAGCAACACGGGTTCAGGTA | |
| TIMP1 | TIMP1-F | GCCATCGCCGCAGATC |
| TIMP1-R | GCTATCAGCCACAGCAACAACA | |
| TUBB | TUBB-F | CTGGACCGCATCTCTGTGTACTAC |
| TUBB-R | GACCTGAGCGAACAGAGTCCAT | |
| VEGFA | VEGFA-F | CTACCTCCACCATGCCAAGTG |
| VEGFA-R | GCGCTGATAGACATCCATGAAC |