Fig 2. Experimental design.

A preliminary study optimized anaesthesia dosing and evaluated autonomous breathing after mechanical ventilation for one hour. Since no evidence of gross pathology damage was found, the pre-clinical trial was performed. The trial included a total of eight animals whose autonomous breathing was evaluated after mechanical ventilation for six hours. During the trial, the results from the first group of four individuals were done and since no post-mortem evidence of damage to internal organs was found, the responses from a second group of four individuals were further studied.