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. 2022 Feb 18;16(2):e0009850. doi: 10.1371/journal.pntd.0009850

Table 3. Decision algorithm for classification of samples based on the data obtained with the new multiplex qPCR.

Results Classification
18S rRNA negative Extraction failure (repeat extraction)
18S rRNA Cp between 13 and 32 Valid reaction (proceed with classification)
RLEP < 34.5 and 16S rRNA < 35.5 M. leprae detected
RLEP < 34.5 and 16S rRNA ≥ 35.5 Equivocal (mark patient for new sample collection and testing)
RLEP ≥ 34.5 M. leprae undetected