Table 3. Decision algorithm for classification of samples based on the data obtained with the new multiplex qPCR.
| Results | Classification |
|---|---|
| 18S rRNA negative | Extraction failure (repeat extraction) |
| 18S rRNA Cp between 13 and 32 | Valid reaction (proceed with classification) |
| RLEP < 34.5 and 16S rRNA < 35.5 | M. leprae detected |
| RLEP < 34.5 and 16S rRNA ≥ 35.5 | Equivocal (mark patient for new sample collection and testing) |
| RLEP ≥ 34.5 | M. leprae undetected |