Fig 2. miR-142 is required for maintenance of T_reg cell–mediated immune tolerance.

(A) Left panel, FACS analysis of splenic lymphocytes from 12-week-old Foxp3^Cre and Foxp3^CremiR-142^fl/fl mice with anti-CD4 and anti-Foxp3 specific antibodies. Numbers indicate percentage of CD4⁺Foxp3⁺ T_reg cells in the gate. Right panel, frequency and absolute number of T_reg cells in Foxp3^Cre and Foxp3^CremiR-142^fl/fl spleens (n = 3 per group). (B) FACS analysis of splenic CD4⁺ and CD8⁺ T cells from 8- to 10-week-old Foxp3^Cre and Foxp3^CremiR-142^fl/fl mice with anti-CD44 and anti-CD62L specific antibodies. Numbers indicate percentage of cells in the quadrants. (C) Frequency of naive (CD44⁻CD62L⁺) and activated (CD44⁺CD62L⁻) splenic CD4⁺ (top) and CD8⁺ (bottom) T lymphocytes isolated from 8- to 10-week-old Foxp3^Cre and Foxp3^CremiR-142^fl/fl mice (n = 6 per group). (D) Intracellular FACS analysis of IFNγ production in splenic CD4⁺ (top) and CD8⁺ (bottom) T cells. Numbers indicate percentage of IFNγ⁺ T cells in the gate. (E) Frequency of IFNγ expressing splenic CD4⁺ and CD8⁺ T cells isolated from 8- to 10-week-old Foxp3^Cre and Foxp3^CremiR-142^fl/fl mice (n = 4 per group). Results are shown as mean ± SD. P values were calculated using 2-tailed Student t test. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. The underlying numerical raw data can be found in S1 Data file. The underlying flow cytometry raw data can be found at the Figshare repository. FACS, fluorescence activated cell sorting; IFNγ, interferon gamma; SD, standard deviation; T_reg, regulatory T.