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. 2022 Feb 18;20(2):e3001552. doi: 10.1371/journal.pbio.3001552

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© 2022 Wang et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — (A) Left panel, FACS analysis of splenic T_reg cells from 12- to 15-week-old Foxp3^Cre (WT), Foxp3^CremiR-142^fl/fl (KO), and Foxp3^CremiR-142^fl/flIfng^−/− (DKO) mice (n = 4 per group). Foxp3⁺CD4⁺ T_reg cells are gated and numbers indicate the percentage of cells in the gate. Right panel, frequency of CD4⁺Foxp3⁺ T_reg cells in Foxp3^Cre (WT), Foxp3^CremiR-142^fl/fl (KO), and Foxp3^CremiR-142^fl/flIfng^−/− (DKO) spleens. (B) Kaplan–Meier survival curves for Foxp3^Cre (red line; n = 27), Foxp3^CremiR-142^fl/fl (blue line; n = 27), and Foxp3^CremiR-142^fl/flIfng^−/− (green line; n = 12) mice. (C) Body weight comparison of 7- to 9-week-old female Foxp3^Cre (WT, red bar, n = 7), Foxp3^CremiR-142^fl/fl (KO, blue bar, n = 6), and Foxp3^CremiR-142^fl/flIfng^−/− (DKO, green bar, n = 3) mice. (D) Representative images of spleen and inguinal lymph nodes from 14-week-old Foxp3^Cre, Foxp3^CremiR-142^fl/fl, and Foxp3^CremiR-142^fl/flIfng^−/− mice. (E) Spleen weights in 12- to 15-week-old Foxp3^Cre (WT, red bar, n = 4), Foxp3^CremiR-142^fl/fl (KO, blue bar, n = 3), and Foxp3^CremiR-142^fl/flIfng^−/− (DKO, green bar, n = 4) mice. (F) Thymus weights (1 lobe) in 6- to 8-week-old Foxp3^Cre (WT, red bar), Foxp3^CremiR-142^fl/fl (KO, blue bar), and Foxp3^CremiR-142^fl/flIfng^−/− (DKO, green bar) mice (n = 3 per group). (G) HE staining of liver tissue sections from Foxp3^Cre (WT), Foxp3^CremiR-142^fl/fl (KO), and Foxp3^CremiR-142^fl/flIfng^−/− (DKO) mice. Note diminished infiltration of leukocytes into liver tissue around portal vein area in DKO mice. Scale bar, 50 μm; p, portal vein. (H) Left panel, FACS analysis of CD44 and CD62L expression in splenic CD4⁺ T cells from 7- to 15-week-old Foxp3^Cre, Foxp3^CremiR-142^fl/fl, and Foxp3^CremiR-142^fl/flIfng^−/− mice. Numbers indicate percentage of cells in the quadrants. Right panel, frequency of CD44⁻CD62L⁺ naive CD4⁺ T cell in Foxp3^Cre (WT, n = 7), Foxp3^CremiR-142^fl/fl (KO, n = 6), and Foxp3^CremiR-142^fl/flIfng^−/− (DKO, n = 7) spleens. Results are shown as mean ± SD. P values were calculated using 2-tailed Student t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. WT, Foxp3^Cre; KO, Foxp3^CremiR-142^fl/fl; DKO, Foxp3^CremiR-142^fl/flIfng^−/−. The underlying numerical raw data can be found in S1 Data file. The underlying flow cytometry raw data can be found at the Figshare repository. DKO, double knockout; FACS, fluorescence activated cell sorting; HE, hematoxylin–eosin; IFNγ, interferon gamma; KO, knockout; SD, standard deviation; WT, wild-type.