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. 2022 Mar 3;20(3):e3001530. doi: 10.1371/journal.pbio.3001530

Fig 5. Astrocytes have broader response field width and a different distribution of field position compared to neurons.

Fig 5

(A, B) ROIs corresponding to simultaneously recorded GCaMP6f-labeled astrocytes (A) and jRCaMP1a-labeled neurons (B) in the CA1 pyramidal layer. ROIs are color coded according to response field and place field center along the virtual corridor, respectively. Scale bar: 20 μm. (C) Normalized calcium responses as a function of position for astrocytic ROIs (left) and neuronal ROIs (right) that contain a significant amount of spatial information (astrocytic ROIs, N = 76 ROIs with reliable spatial information out of 341 total ROIs; neuronal ROIs, N = 335 ROIs with reliable spatial information out of 870 total ROIs, data from 11 imaging sessions in 7 animals). Responses are ordered according to the position of the center of the response field for astrocytes and place field for neurons. Vertical scale bar, 20 ROIs. (D) Distribution of astrocytic response field position (black line) and neuronal place field position (gray line, p = 5E-4, Kolmogorov–Smirnov test for comparison between astrocytic and neuronal distribution). (E) Distribution of astrocytic response field width (black line) and neuronal place field width (gray line, median width of astrocytic response field: 42 ± 22 cm, N = 76; median width of neuronal place field: 37 ± 10 cm, N = 335, p = 2E-5, Wilcoxon rank sums test for comparison between astrocytic and neuronal distribution). (F, G) The inset shows astrocytic ROIs (green) and neuronal ROIs (pink). For all pairs, the distance (d0, d1, dn) between the center of an astrocytic ROI and the center of a neuronal ROI, both containing reliable spatial information, is computed. Pairwise Pearson correlation (F) and difference between response field position for astrocyte–neuron ROI pairs (G) as a function of pair distance. In (F,G) Data are expressed as mean ± SEM. Data are from 11 imaging sessions in 7 animals (see also S15 Fig). The data presented in this figure can be found in S1 Data. ROI, region of interest; SEM, standard error of the mean.