Cell line (Homo sapiens) |
Hep3B |
ATCC |
Cat#: HB-8064;RRID:CVCL_0326
|
|
Cell line (H. sapiens) |
HepG2 |
ATCC |
Cat#: HB-8065; RRID:CVCL_0027
|
|
Cell line (H. sapiens) |
Huh6 |
RCB |
Cat#: RCB1367; RRID:CVCL_4381
|
|
Cell line (H. sapiens) |
Huh7 |
JCRB |
Cat#: JCRB0403; RRID:CVCL_0336
|
|
Cell line (H. sapiens) |
MHCC97H |
Zhongshan Hospital |
RRID:CVCL_4972
|
Liver Cancer Institute of Zhongshan Hospital (Shanghai, China) |
Cell line (H. sapiens) |
PLC/PRF/5 |
ATCC |
Cat#: CRL-802;RRID:CVCL_0485
|
|
Cell line (H. sapiens) |
SNU398 |
ATCC |
Cat#: CRL-2233; RRID:CVCL_0077
|
|
Cell line (H. sapiens) |
SNU449 |
ATCC |
Cat#: CRL-2234; RRID:CVCL_0454
|
|
Cell line (H. sapiens) |
SNU475 |
ATCC |
Cat#: CRL-2236;RRID:CVCL_0497
|
|
Cell line (H. sapiens) |
SK-Hep1 |
ATCC |
Cat#: HTB-52; RRID:CVCL_0525
|
|
Cell line (H. sapiens) |
LX2 |
ATCC |
Cat#: SCC064;RRID:CVCL_5792
|
|
Chemical compound, drug |
Homoharringtonine |
Selleck Chemicals |
S9015 |
|
Antibody |
Anti-HSP90 (Mouse monoclonal) |
Santa Cruz Biotechnology |
Cat#: sc-13119;RRID:AB_675659
|
WB (1:5000) |
Antibody |
Anti-α-SMA (Mouse monoclonal) |
Sigma-Aldrich |
Cat#: A5228; RRID:AB_262054
|
WB (1:2000)IF (1:200) |
Antibody |
Anti-Collagen I (Rabbit polyclonal) |
ProteinTech |
Cat#: 14695-1-AP; RRID:AB_2082037
|
WB (1:2000)IF (1:200) |
Sequence-based reagent |
ACTA2_F |
This paper |
PCR primer |
5′GACAATGGCTCTGGGCTCTGTAA3′ |
Sequence-based reagent |
ACTA2_R |
This paper |
PCR primer |
5′CTGTGCTTCGTCACCCACGTA3′ |
Sequence-based reagent |
COL1A1_F |
This paper |
PCR primer |
5′TCCTGGTCCTGCTGGCAAAGAA3′ |
Sequence-based reagent |
COL1A1_R |
This paper |
PCR primer |
5′CACGCTGTCCAGCAATACCTTGA3′ |
Software, algorithm |
R software, version 3.6.0 |
https://cran.r-project.org/
|
RRID:SCR_001905
|
|
Software, algorithm |
ImageJ, version 1.53k |
http://imagej.net/
|
RRID:SCR_003070
|
|
Software, algorithm |
Combenefit, version 2.02 |
https://sourceforge.net/projects/combenefit/
|
|
|