| Strain, strain background (Escherichia coli) |
BL21(DE3) |
Novagen |
69,450 |
Chemically competent cells |
| Recombinant DNA reagent |
pET(11a)-RFC[2 + 3 + 4] (plasmid) |
Finkelstein et al., 2003
|
|
Expression plasmid |
| Recombinant DNA reagent |
pLANT-2/RIL[1 + 5] (plasmid) |
Finkelstein et al., 2003
|
|
Expression plasmid |
| Recombinant DNA reagent |
pRS413-RFC1(plasmid) |
This study |
|
Plasmid for yeast expression of Rfc1 from endogenous promotor |
| Strain, strain background (S. cerevisiae) |
BY4743his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15 ura3Δ0/ura3Δ0 ∆rfc1::KanMX4/RFC1 (YOR217W) |
Dharmacon |
YSC1055 (22473) |
Yeast Heterozygous Collection |
| Software, algorithm |
RELION |
doi:10.7554/eLife.42166
|
Relion 3.0.2 |
|
| Software, algorithm |
cisTEM |
doi:10.7554/eLife.35383
|
cisTEM-1.0.0-beta |
https://cistem.org/software
|
| Software, algorithm |
Ctffind |
doi:10.1016/j.jsb.2015.08.008
|
Ctffind 4.1 |
|
| Software, algorithm |
UCSF Chimera |
UCSF, doi:10.1002/jcc.20084
|
|
http://plato.cgl.ucsf.edu/chimera/
|
| Software, algorithm |
ChimeraX |
UCSF, doi:10.1002/pro.3943
|
ChimeraX-1.2 |
https://www.cgl.ucsf.edu/chimerax/
|
| Software, algorithm |
COOT |
doi:10.1107/S0907444910007493
|
Coot-0.9.4 |
http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
|
| Software, algorithm |
Phenix |
doi:10.1107/S0907444909052925
|
Phenix-dev-3699 |
https://phenix-online.org
|
| Software, algorithm |
PyMOL |
PyMOL Molecular Graphics System, Schrodinger LLC |
|
https://www.pymol.org/
|
| Software, algorithm |
GraphPad PRISM |
GraphPad |
GraphPad PRISM 9.2.1 |
http://www.graphpad.com/
|
| Other |
Pyruvate kinase |
Calzyme |
107A0250 |
|
| Other |
Lactate Dehydrogenase |
Worthington Biochemical Cooperation |
LS002755 |
|
| Other |
Phosphoenol-pyruvic acid monopotassium salt |
Alfa Aesar |
B20358 |
|
