| Magnification |
130,000 |
81,000 |
| Voltage (keV) |
300 |
300 |
| Cumulative exposure(e−/Å 2) |
49–51 |
40 |
| Detector |
K2 Summit |
K3 |
| Pixel size (Å) |
1.059 |
1.06 |
| Defocus range (μm) |
−1.1 to −2.4 |
−1.2 to −2.3 |
| Micrographs used (no.) |
6109 |
4499 |
| Initial particle images (no.) |
954,291 |
1,331,440 |
| Symmetry |
C |
|
Class name
|
Autoinhibited1
|
Autoinhibited2
|
Autoinhibited3
|
Open1
|
Open2
|
DNA-open
|
DNA-closed
|
| Final refined particles (no.) |
55,308 |
68,227 |
60,036 |
46,069 |
63,752 |
46,300 |
76,270 |
| Applied B factor (Å2) |
−100 |
−159.352 |
−163.938 |
−100 |
−106.457 |
−105.857 |
−105.313 |
| Map resolution(Å, FSC 0.143) |
3.8 |
3.9 |
4.0 |
4.0 |
3.5 |
3.4 |
3.3 |
| Model-Map CC_mask |
0.78 |
0.77 |
0.77 |
0.76 |
0.78 |
0.79 |
0.77 |
| Bond lengths (Å), angles (°) |
0.002,0.585 |
0.002,0.561 |
0.002,0.558 |
0.002,0.574 |
0.002,0.542 |
0.002,0.518 |
0.002,0.523 |
| Ramachandran outliers, allowed, favored |
0.00,3.16, 96.84 |
0.00,3.11, 96.89 |
0.00,2.89, 97.11 |
0.00,3.08, 96.92 |
0.00,3.38, 96.62 |
0.00,2.23, 97.77 |
0.00,2.16, 97.84 |
| Poor rotamers (%),MolProbity score, Clashscore (all atoms) |
0.00,1.68,9.05 |
0.00,1.68,9.42 |
0.00,1.68,9.95 |
0.00,1.67,9.26 |
2.01,1.91,8.67 |
1.09,1.54,8.44 |
1.09,1.55,9.18 |
| Accession number,EMDB, PDB |
25568,7THJ |
25569,7TIC |
25614,7THV |
25615,7TKU |
25753,7TI8 |
25616,7TIB |
25617, 7TID |
