Table 4. DNA sequences.

Template name	Sequence	Primer name	Sequence	Name used in assay
Template30-20-A	TTTTTTTTTTAATGTACTCGTAGTGTCTGC	Primer20-3’abasic	GCAGACACTACGAGTACAT/3dSp/	p/t-DNA 3'-abasic
		Primer20-3’-T-phosphate	GCAGACACTACGAGTACATT/3Phos/	p/t-DNA 3’ PO4
		Primer20-3’-T-RNA	rGrCrArGrArCrArCrUrArCrGrArGrUrArCrArUrU	RNA primer/DNA template
		Primer20-3’-riboT	GCAGACACTACGAGTACATrU	p/t-DNA 3’ ribo
		Primer20-3’-T	GCAGACACTACGAGTACATT	p/t-DNA
		Primer20-2AP-0	GCAGACACTACGAGTACAT/32AmPu/	p/t-AP, P = 1
		Primer20-2AP-2	GCAGACACTACGAGTAC/i2AmPr/TA	p/t-AP, P = 3
Template30-T-1	TTTTTTTTTTTTTGTACTCGTAGTGTCTGC-3’	Primer20-2AP-1	GCAGACACTACGAGTACA/i2AmPr/A	p/t-AP, P = 2
Template30-20-2AP	TTTTTTTTTT/i2AmPr/ATGTACTCGTAGTGTCTGC-3’	Primer20-3’-T	GCAGACACTACGAGTACATT	p/t-AP, t = 1
Template20-5’-A	AATGTACTCGTAGTGTCTGC	Primer20-3’-T	GCAGACACTACGAGTACATT	Blunt DNA
		Primer 20–3'-T-10ext	GCAGACACTACGAGTACATTTTTTTTTTTT	3' overhang DNA
Template30-20-A-3’T	AATGTACTCGTAGTGTCTGCTTTTTTTTTT	Primer 20–3'-T-10ext	GCAGACACTACGAGTACATTTTTTTTTTTT	3' overhang dumbbell DNA
		polyT 20	TTTTTTTTTTTTTTTTTTTT	ssDNA (poly T)