Table 1. Composition of juices employed for the in vitro digestion model [39].
| Saliva
(pH 6.8 ± 0.1) |
Gastric juice
(pH 1.3 ± 0.1) |
Duodenal juice
(pH 8.1 ± 0.1) |
Bile juice
(pH 8.2 ± 0.1) |
Sodium carbonate
solution |
|---|---|---|---|---|
| 896 mg KCl | 2752 mg NaCl | 7012 mg NaCl | 5259 mg NaCl | 84.7 g NaHCO3 |
| 200 mg KSCN | 306 mg NaH2PO4 H2O | 3388 mg NaHCO3 | 5785 mg NaHCO3 | Milli-Q water |
| 1021 mg NaH2PO4 H2O | 824 mg KCl | 80 mg KH2PO4 | 376 mg KCl | |
| 570 mg Na2SO4 | 302 mg CaCl2 | 564 mg KCl | 150 ml HCl (37%) | |
| 298 mg NaCl | 306 mg NH4Cl | 50 mg MgCl2 6H2O | 250 mg urea | |
| 1694 mg NaHCO3 | 6.5 ml 37% HCl | 180 ml HCl (37%) | 167.5 mg CaCl2 | |
| 200 mg urea | 650 mg glucose | 100 mg urea | 1.8 g BSA* | |
| 290 mg amylase* | 20 mg glucuronic acid | 151 mg CaCl2 | 30 g bile* | |
| 15 mg uric acid | 85 mg urea | 1 g BSA* | Milli-Q water | |
| 25 mg mucin* | 330 mg
glucosaminehydrochloride |
9 g pancreatin* | ||
| Milli-Q water | 1 g BSA* | 1.5 g lipase* | ||
| 2.5 g pepsin* | Milli-Q water | |||
| 3 g mucin* | ||||
| Milli-Q water |
*Protein components included in the “digestion with proteins” but not included in the “digestion without proteins”.