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. 2022 Feb 27;9:113–126. doi: 10.2147/JHC.S349369

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© 2022 Xu et al.

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MiR-128-3p regulates c-Met by binding to its 3’-UTR. (A) The wild-type (WT) and mutated (MT) luciferase vectors containing the 3’-UTR of human c-Met gene were constructed. (B) Huh7 cells were co-transfected with the WT vector and negative control (NC) or each miRNA mimics as indicated. Mock-treated cells served as controls. (C) The expression level of each miRNA in Huh7 and Huh7-LR was detected by RT-qPCR and their fold changes (Huh7-LR/Huh7) are plotted. (D) The miR-128-3p binding site on the 3’-UTR of c-Met is highly conserved across all the species. (E) Huh7 cells were co-transfected with the WT vector and miR-128-3p mimics at different concentrations. (F) Huh7 cells were co-transfected with WT or MT vectors, and NC, miR-128-3p mimics or antagomiR-128-3p oligonucleotides. Luciferase activities were measured and normalized to mock-treated cells. **P<0.001.