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. 2022 Feb 17;10:845461. doi: 10.3389/fcell.2022.845461

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Copyright © 2022 Filova, Bohuslavova, Tavakoli, Yamoah, Fritzsch and Pavlinkova.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ectopic hair cells differentiate outside the sensory epithelia in the Neurod1CKO inner ear. Representative images of whole mount immunolabeling with MyosinVIIa (MyoVII, a marker of hair cells) and tubulin (neuronal fibers) show hair cells in the saccule and rows of hair cells forming the organ of Corti in the Neurod1CKO cochlea. Ectopic MyoVIIa positive cells (eHCs) are found near the saccule and the aberrant cochlear ganglion (a dotted line delineates the area). Larger magnification images show eHCs entangled with neuronal fibers, and some cells are double-labeled for tubulin and MyoVIIa. Scale bars: 100 µm (A,B); 20 μm (A’,B’).