FIGURE 7.

Auditory afferent synapses are reduced in the anteroventral cochlear nucleus of Neurod1CKO adult mice. (A) Representative confocal images of anti-parvalbumin labeled spherical bushy cells and anti-VGLUT1 labeled auditory-nerve endings, endbulbs of Held, show a loss of auditory afferent synapses in Neurod1CKO that wrap the soma of bushy cells. (B) Quantification of spherical bushy cells (n = 4 mice/two sections/genotype) and VGLUT1⁺ synapses per the parvalbumin⁺ area (n = 4 mice/two sections/three cells per section/genotype). The values represent means ± SD, t-test; ns, not significant; ****p ≤ 0.0001. Scale bars: 20 µm. HS, Hoechst nuclear staining.