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. 2022 Feb 14;7(3):397–410. doi: 10.1038/s41564-022-01059-2

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© The Author(s) 2022, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, Top: cryoET slices of purified wild-type AlgoCIS and different deficient mutants showing that the fractions of empty AlgoCIS particles are significantly increased in mutants (wild-type, n_total = 2,620; Cgo1/Cgo2⁻, n_total = 543; Cgo1⁻, n_total = 1,383; Cgo2⁻, n_total = 1,104). Bottom left: representative filled and empty AlgoCIS particles. Bottom right: quantification (blue, filled; red, empty). The individual empty AlgoCIS particles are highlighted by red arrowheads, whereas one filled AlgoCIS particle is highlighted by a blue arrowhead. Tomographic slices are 10.8 nm thick. Scale bars, 50 nm. b, Central volume slices showing the sub-tomogram averages of wild-type AlgoCIS (left) and Cgo1/Cgo2⁻ (middle). The difference map (right) between wild-type and Cgo1/Cgo2⁻ mutant shows additional densities in cyan (corresponding to cargo proteins) and the remaining parts in white. Scale bars, 10 nm.

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