Figure 3.

Construction and characterization of MTBVAC disA and MTBVAC cnpB mutants

(A) Schematic representation of the construction of the MTBVAC disA mutant indicating the primers used to confirm the genetic deletion of disA by PCR. The lower panel shows PCR bands of ΔdisA::Km colonies indicative of a specific allelic exchange. (B) Schematic representation of the construction of the MTBVAC cnpB mutant indicating the primers used to confirm the genetic deletion of cnpB by PCR. The lower panel shows PCR bands of ΔcnpB::Km colonies indicative of a specific allelic exchange. (C) Production of c-di-AMP in MTBVAC and its disA and cnpB mutant derivatives. (D) Secretion of c-di-AMP in MTBVAC and its disA and cnpB mutant derivatives. n.d., not detected. Data are the mean and standard deviation from three biological replicates.