Figure 4.

IFNβ and IL-1β responses during THP-1 macrophage infections with different MTBVAC derivatives

(A) IFNβ produced by macrophages infected with the vaccines MTBVAC, its disA and cnpB mutants, and BCG Pasteur. M. tuberculosis MT103 and H37Rv strains and the M. tuberculosis ΔRD1 mutant served as positive and negative controls of IFNβ production, respectively. (B) IL-1β produced after macrophage infection with the strains denoted in (A). Bars represent the mean and standard deviation from three cellular infections. Statistical analysis was performed using one-way ANOVA followed by Turkey’s post-test. Asterisks indicate the following p values: ∗ 0.05 > p > 0.01; ∗∗ 0.01 > p > 0.0005; ∗∗∗∗ 0.0001 > p.