Figure 5.

Characterization of the impact of differential c-di-AMP production on MTBVAC vaccine attenuation and efficacy

(A) Bacterial loads in the lungs of SCID mice after 4 weeks infected with BCG Pasteur, MTBVAC, and the MTBVAC disA and cnpB mutants. (B) Protective efficacy of BCG Pasteur, MTBVAC, and the MTBVAC disA and cnpB mutants in the C3H/HeNRj mouse model. Mice were immunized with the previously mentioned strains, left unvaccinated as a control, and challenged 8 weeks later with the M. tuberculosis Beijing W4 strain. Bacterial loads in the lungs were enumerated 4 weeks after the challenge as a measure of vaccine efficacy. All data are mean ± SEM. Statistical analysis was performed by one-way ANOVA and the Bonferroni post hoc test.