Table 2.
Primers used in this study
| Primers | Sequence 5–3 |
|---|---|
| cnpB-P1-Fw | GGGTGCGGCAAGCGGGTAGAGGTC AGCTTTGCCGCGCCGGGTGT AGGCTGGAGCTGCTTC |
| cnpB-P2-Rv | GCGTCGTCGATCGAGCCGG TGGTCGTATACCCCGCGGC CACATATGAATATCCTCCTTAGT |
| KO-cnpB-Fw | GGGCGTTGTTCCGGTCTTCG |
| KO-cnpB-Rv | CCTAGCCCTAACGCCGCTG |
| Confirm-KO-cnpB-Fw | GCGGTGATCATCGGTTTCAATGTGCG |
| Confirm-KO-cnpB-Rv | CAACGCACTCGCCACCGTC |
| cnpB-I1 | GGGCGATCTAACTGATTCCGGGC |
| cnpB-I2 | CGACCTCCTTGAACACCGCCG |
| disA-P1-Fw | GCACGCTGTGACTCGTCCGACCCTGC GTGAGGCTGTCGCCGTGTAGGCT GGAGCTGCTTC |
| disA-P2-Rv | CGTCCGACAGGGCGTCCAGTTCGT CCAGGGTGGCATTGATCATATGAAT ATCCTCCTTAGT |
| KO-disA-Fw | CTCGCCGCACAGTCCGAC |
| KO-disA-Rv | GATCGCGGTGGCCATCGTCATC |
| Confirm-KO-disA-Fw | GCGCGCTCTATGTCTCTGGTGAG |
| Confirm-KO-disA-Rv | CGTTTGCGTGACCTGGCCCTAC |
| disA-I1 | GATGGTGGCTTCTCCCTCGATGTC |
| disA-I2 | CACCACCGTCATCACATCGCG |
| P1-inv | GAAGCAGCTCCAGCCTACAC |
| P2-inv-long | CTTCGGAATAGGAACTAAG GAGGATATTCATATG |