FIGURE 2.

PB1 K229R that reduces susceptibility to T-705 also reduces susceptibility to SMeFM. MDCK cells were infected with wild type (PR8 strain) (A) or mutant virus containing NA E119V (B), NA R293K (C), and H275Y (D) (N1 numbering; low susceptibility to neuraminidase inhibitors), PA I38T (E) (low susceptibility to baloxavir marboxil), or PB1 K229R (F) (low susceptibility to T-705) at an MOI of 0.01 and incubated with SMeFM or indicated compound for 24 h. The virus titer in the supernatant was determined by plaque assay. BXM: baloxavir marboxil, Ose: oseltamivir, and Zan: zanamivir. The graph indicates average values with standard deviations from three independent experiments. The circles indicate the titer of each experiment. P-values were calculated by Dunnett’s multiple comparison test. An asterisk indicates P-value less than 0.05.