FIGURE 3.

Anti-influenza virus activity of T-705 does not affect that of SMeFM. (A) Inhibition of IAV propagation by the combination of SMeFM and T-705. MDCK cells were infected with wild type virus (WSN strain) at an MOI of 0.01 and incubated with the indicated concentration of SMeFM and T-705 for 24 h. The virus titer in the supernatant was determined by plaque assay. (B) Isobologram analysis of the combination of SMeFM and T-705. The individual doses of SMeFM and T-705 to achieve 99% growth inhibition (IC₉₉) were plotted on the X- and Y-axes. The line of additivity on the isobologram represents the 99% growth inhibition level of each drug. The combination index (CI) is represented by the X symbol above (CI > 1, indicating antagonism between drugs) or below (CI < 1, indicating synergy) the line (CI = 1, indicating additive effect).