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Pre-treatment of SMeFM enhances the inhibitory effect of SMeFM on viral protein synthesis. MDCK cells were pretreated with 3 μM of T-705 or 1 μM of SMeFM for 24 h. The pretreated cells were infected with the influenza A virus (WSN strain) at an MOI of 1. The cells were collected and lysed at 8 hpi, and NP and M1 were detected by western blotting. D: DMSO, T: T-705, S: SMeFM.
