FIGURE 6.

Adenine to cytosine and uracil to guanine mutations are accumulated in viral genome RNA by the treatment of SMeFM. The mutation rate from treated infected cells treated with DMSO (A), T-705 (B), or SMeFM (C) was shown. The mutation ratio was calculated from the number of polymorphisms of each nucleotide and the total number of polymorphisms (DMSO: 40,640, T-705: 57,923, and SMeFM: 33,161) detected by amplicon-seq. A chi-square test was used to determine the statistical significance of the differences of each mutation type relative to the DMSO control. Statistically significant increases in mutation rates are highlighted by shading.