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. 2022 Feb 18;13:802671. doi: 10.3389/fmicb.2022.802671

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Copyright © 2022 Takizawa, Takada, Umekita, Igarashi and Takahashi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SMeFM triphosphate is not incorporated into synthesized RNA. (A) In vitro RNA synthesis from vRNPs with SMeFM and SMeFM triphosphate (SMeFM TP). In vitro RNA synthesis assay was performed with SMeFM or SMeFM TP at 30°C for 30 min. Synthesized RNAs were separated by UREA-PAGE. (B) Limited elongation assay with SMeFM TP. In vitro RNA synthesis assay was performed with or without UTP, ATP, or SMeFM. Synthesized RNAs were separated by UREA-PAGE. The expected lengths of limited elongation products without UTP were 12 nt for segments 1, 3, and 7, 13 nt for segment 5, 14 nt for segment 6, 18 nt for segment 4, and 19 nt for segment 2.