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. 2022 Feb 18;13:802671. doi: 10.3389/fmicb.2022.802671

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Copyright © 2022 Takizawa, Takada, Umekita, Igarashi and Takahashi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SMeFM treatment reduces the cellular ATP pool. (A) The relative concentration of ATP in cells treated with SMeFM. The cellular ATP concentration was measured as luminescence count and was normalized by the cell number. The relative ATP concentration was calculated by dividing the normalized luminescence count by that of cells without SMeFM treatment which is set as 1. The graph indicates average values with standard deviations from four independent experiments. P-values were calculated by Dunnett’s multiple comparison test. (B) The concentration of NTPs in cells treated with SMeFM. MDCK cells were treated with SMeFM or T-705 at indicated concentrations, and NTP concentration was analyzed by HPLC. The graph indicates average values with standard deviations from three independent experiments. The circles indicate the value of each experiment. P-values were calculated by Dunnett’s multiple comparison test.