Figure 3.

Impact of TBF on supra-threshold excitability in DG granule cells

For all panels, the Control (black) group (n = 10) corresponds to experiments where no protocol was applied through the 45-min period of the experiment, and TBF (red) group (n = 32) is for neurons subjected to TBF (same population of cells from Figure 2). For the TBF group, “Baseline” measurements were obtained before TBF and “TBF” measurements were after TBF

(A) Population data representing changes in the action potential firing frequency at the beginning (empty circles) and end (filled circles) of the experiment, for six values of current injection

(B) Summary statistics (mean ± SEM) of action potential firing frequency plotted as a function of injected current amplitude for the Control group. ∗p < 0.05; ∗∗p <0.005. Student’s t test

(C and D) Same as (A–B) for the TBF group where the cells were subjected to TBF. For each of the five (50, 100, 150, 200, and 250 pA) current injections, there was no significant interaction between time (0 vs. 45 min) and protocol (Control vs. TBF) factors when assessed with two-way mixed ANOVA

(E) Plots comparing percentage change in various supra-threshold measurements from their initial values to end of experiment values, for both Control and TBF experiments. The list of symbols and corresponding measurements is enumerated in Table S1. The Wilcoxon rank-sum test was employed for p-value calculation in panel E, to compare percentage changes in the Control vs. TBF group. Details of statistics associated with these measurements are provided in Table S2