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. 2021 Dec 16;36(1):10–21. doi: 10.7555/JBR.35.20200207

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Copyright and License information: Journal of Biomedical Research, CAS Springer-Verlag Berlin Heidelberg 2022

This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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c-Met CAR-T cells produced IFN-γ and IL-2 upon stimulation with HepG2 and shMet-HepG2 cells.

A and B: The secretion of IFN-γ (A) and IL-2 (B) of the engineered T cells following coculture with HepG2 cells for 24 hours at a 10:1 ratio was determined by ELISA. C and D: The secretion of IFN-γ (C) and IL-2 (D) of the engineered T cells following 24 hours coculture with shMet-HepG2 cells at a 10:1 ratio was determined by ELISA. Data were the mean±SEM from triplicates. Statistical analyses were performed by one-way ANOVA for comparisons between multiple groups with a single variable. ^*P<0.05. IFN-γ: interferon-gamma; IL-2: interleukin-2; ELISA: enzyme-linked immunosorbent assay.