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. 2022 Mar 3;13:1137. doi: 10.1038/s41467-022-28722-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Processing of CT N-glycans on Rituximab. Mass spectrometry of Rituximab treated with a no enzyme (negative control) b EndoE-GH18L c EndoE-GH18L_E186Q d EndoE-GH18L + EndoE-GH18L_E186Q e EndoE-GH20 f EndoE-GH20_E662Q g EndoE-GH20 + EndoE-GH20_E662Q h EndoS2 (positive control) i EndoE j EndoE-GH18L + EndoE-GH20 k EndoE_E186Q l EndoE_E186Q + EndoE-GH18L m EndoE_E662Q n EndoE_E662Q + EndoE-GH20. The peaks corresponding to intact Rituximab are numbered based on the glycoforms found in each heavy chain. The retention time for Rituximab was 2.4 min. For mass deconvolution, the following parameters were used in the BioConfirm software; 2000–7000 m/z and 14.4–14.8 kDa. The theoretical and observed mass of each annotated peak are in Supplementary Table 4.