Fig. 4. A.27 shows a mutational pattern in its viral spike gene that resembles other VOCs and VOIs.

a Schematic overview of the SARS-CoV-2 spike gene and the non-synonymous mutations found in more than 75% of A.27 sequences (n = 1386) compared to the S of Wuhan-Hu-1 sequence (NC_045512.2). NTD–N-terminal domain, RBD–receptor-binding domain, RBM–receptor-binding motif, FP–fusion peptide, HR1/2–heptad repeat region, TM–transmembrane domain, CT–C-terminal domain. b The spike protein of SARS-CoV-2 (PDB accession number: 6vxx) with the mutations displayed in (a) is shown in surface presentation. c Comparison of spike mutations present in A.27 and the different VOCs and VOIs. The mutation frequencies of the different lineages were downloaded from outbreak.info⁷⁰ (accessed on 2021-07-13) and the A.27 mutation frequencies replaced with the frequencies calculated based on the 1386 sequences used in this study. The heatmap was visualized with the R pheatmap package. Source data are provided as a Source Data file.