Fig. 2. Serine/Threonine Kinase (STK) activity in untreated retinal explants.

Organotypic retinal explants derived from wild-type (WT) and rd1 mice (WT, n = 5; rd1, n = 8) were maintained in culture medium from P5 till P11. The kinase activity of their lysates was measured on PamChip® Serine/Threonine kinase (STK) arrays. a Violin plot showing the global phosphorylation of the peptides on PamChip® STK array as Log₂ signal intensity and their intensity value distribution, when comparing WT to rd1 explants. The thick line connects the average values of each group. b Volcano plot representing Log Fold Change (LFC) and −Log₁₀ p-value for peptide phosphorylation. Red dots indicate significantly changed phosphopeptides (p-value < 0.05), black dots represent peptides with no significant alteration in phosphorylation. c The high-ranking kinases were visualized in a kinome phylogenetic tree, where branch and node color are encoded according to the kinase statistic, with values > 0 (in red) representing higher kinase activity in rd1 retinal explants. The node size is encoded by the kinase score, that ranks kinases based on their significance and specificity in terms of sets of peptides used for the corresponding kinase.