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. 2022 Mar 3;12:3560. doi: 10.1038/s41598-022-07187-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Acquisition of serum and whole blood samples from healthy controls and patients. (A) Blood samples were collected from healthy controls and patients (B). One whole blood sample was sent directly for immunophenotyping. Additional blood samples were placed into tubes containing gold-microparticles and incubated at 37 °C for 24 h. After incubation, samples were centrifuged to remove microparticles and blood cells, and serum was decanted. The decanted serum was used for further analysis. In addition, the serum was re-injected into the patients for therapy treatment. *After the second treatment with autologous serum (before they received the third injection) another sample was generated for analysis. CC By Jörg Pekarsky.