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. 2022 Mar 3;12:3500. doi: 10.1038/s41598-022-07394-y

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression after irradiation is fractionation- and time-dependent. (A) Treatment schedule for single dose (SD) and multifractionated (MF) irradiation. MF irradiation was performed with 2 fractions of 1 Gy per day to a total dose of 10 Gy. (B). Heat map of gene expression which was analyzed at 24 h (short term, ST) or 2 months (long term, LT) after the final irradiation dose. Relative gene expression was normalized to the unirradiated control. (C) Venn diagrams showing the number of overlapping or distinct significantly altered genes (fold change ≤ 0.66 or fold change ≥ 1.5, P < 0.05) under the different treatment conditions compared to the unirradiated controls.