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. 2022 Mar 3;12:3500. doi: 10.1038/s41598-022-07394-y

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Radiotherapy induces phosphorylation immediately after the final radiation dose. (A) PC3 cells were irradiated either with a single dose (SD) of 10 Gy or with multifractionated (MF) irradiation of 10 times 1 Gy and incubated for 30 min (short-term, ST) or cultured for 2 months (long-term, LT). Unirradiated cells were used as control. (B) Significant short-term and long-term changes (fold change ≤ 0.66 or fold change ≥ 1.5, P < 0.05) in protein phosphorylation after irradiation of PC3 cells.