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. 2022 Feb 25;54(2):194–205. doi: 10.1038/s12276-022-00735-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The protein expression level of IGF2BP3 in various hematologic tumor cell lines was measured by western blotting. b The knockdown efficiency of IGF2BP3 shRNAs (shIGF2BP3#1 and shIGF2BP3#2) delivered via lentiviral vectors in HL-60 and KG-1 cell lines was confirmed by western blotting. GAPDH was used as the internal reference. c Cell proliferation was measured by a CCK-8 assay at different time points (0, 24, 48, 72, and 96 h) in HL-60 and KG-1 cells after shRNA transduction. d The transduction efficiency after puromycin selection was evaluated by GFP fluorescence imaging in both cell lines. e Flow cytometry (representative images are presented) was used to confirm the induction of apoptosis by IGF2BP3 knockdown. f Western blotting was used to explore apoptosis-related protein levels. The levels of cleaved caspase-3 and Bax were increased but the level of Bcl-2 was decreased under shIGF2BP3 treatment compared with control treatment. g Flow cytometry (representative images are presented) was used to analyze the cell cycle distribution. *P < 0.05; **P < 0.01; ***P < 0.001.