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. 2022 Mar 3;13:1157. doi: 10.1038/s41467-022-28839-y

Fig. 3. Adipocyte-secreted proteins induce chemoresistance in human B-ALL cells.

Fig. 3

Human B-ALL cell lines (REH, Nalm6, and SEM) were preconditioned for 24 h with unconditioned medium (10% RPMI), SCM, or ACM prior to treatment with vehicle (DMSO), methotrexate (MTX), doxorubicin (DOX) or vincristine (VIN) for an additional 2 days (cells remained in CM during chemotherapy treatment). a Representative primary data for REH cells are shown. b The percentage of apoptotic and dead cells on day 3 of culture for each cell line was determined using Annexin-V/ PI staining followed by flow cytometric analysis. e–g B-ALL cells were cultured in RPMI, SCM, or ACM and then treated with MTX as described in a–d (Drug Trt). On day 3 of culture, cells were harvested, and equal numbers of viable cells were cultured in MTX-free medium for an additional 3 days (Recovery). Viable cells were identified and enumerated using trypan-blue exclusion assays. Means ± s.d. are shown (**p < 0.01, ***p < 0.001, and ****p < 0.0001, n = 3 independent experiments, one-way ANOVA with Tukey’s post-test).