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. 2022 Feb 15;54(2):156–168. doi: 10.1038/s12276-022-00731-1

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Fig. 6 — a Hierarchical clustering was conducted with DNA methylation data of intragenic CpG islands of PDX1, EN2, and MSX1, where two distinct subgroups of CRC patients were observed. b, c Kaplan–Meier plots for analyzing the significant differences in b overall survival and c CRC recurrence between the subgroups reveal the prognostic potential of the methylation data of the three biomarkers. The log-rank test was used to compare the significant differences between the two subgroups. One sample was excluded from the analysis of clinical data due to missing clinical data. Additionally, 31 patients were excluded from the recurrence analysis because they were diagnosed with stage IV CRC with metastatic cancers, and differentiating cancer recurrence would be challenging. d qMSP data generated with genomic DNA originating from the tumor and healthy tissues of the seven CRC patients displayed similar patterns to the cohort-specific methylation change analysis in a. The relative methylation levels of intragenic CpG islands of PDX1, EN2, and MSX1 were calculated by dividing the methylation level of the tumor by that of healthy tissue.