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. 2022 Mar 3;12:3556. doi: 10.1038/s41598-022-07451-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Isolation of EVs from organoids established from PDAC patients and healthy controls (HC). (A) Image of representative pancreatic organoid culture (PDAC). (B) Fractions obtained after size exclusion chromatography (SEC) of PDAC organoid supernatants were analyzed for CD9⁺ EVs by flow cytometry and protein levels by measurement at 280 nm. (C) Representative EM images of EVs isolated after SEC on supernatants collected from two healthy control (HC-1 and HC-2) and two PDAC (PDAC-2 and PDAC-4) organoid cultures.