Figure 2.

Activation of NK and T cells in patient with myopericarditis following COVID-19 vaccination. PMBC was isolated from the patient with myopericarditis on days 3 (D3) and 4 (D4) after COVID-19 mRNA vaccination. For flow cytometry analysis, four healthy controls (Healthy) and three individuals who had been vaccinated in the past 2 to 3 days (Vaccine) were used as control. For RT-PCR, three healthy and three vaccinated individuals were used as control. (A) NK cell frequency among PBMC CD45⁺ cells in the patient with COVID-19 vaccine-related myopericarditis. NK cells were gated on CD45⁺CD3^-CD19^-CD56⁺ ( Supplementary Figure 1 ). (B) IFNG, GZMA, and GZMB gene expression in NK cells from the patient with COVID-19 vaccine-related myopericarditis and controls determined by RT-PCR. NK cells were enriched from the PBMC using magnetic beads. (C) Mean fluorescence intensity (MFI) of CD107a expression in PBMC NK cells of the patient with COVID-19 mRNA vaccine-related myopericarditis. (D, E) Flow cytometry plots (D) and frequencies (E) of CD38⁺HLA-DR⁺ and PD-1⁺Ki-67⁺ cells in PBMC CD4⁺ T cells. CD4⁺ T cells were gated on CD45⁺CD3⁺CD4⁺ ( Supplementary Figure 1 ). (F, G) Flow cytometry plots (F) and frequencies (G) of CD38⁺HLA-DR⁺ and PD-1⁺Ki-67⁺ cells in PBMC CD8⁺ T cells. CD8⁺ T cells were gated on CD45⁺CD3⁺CD8⁺ ( Supplementary Figure 1 ). (H, I) Flow cytometry plots (H) and frequencies (I) of CD4⁺ T cell subsets in the patient PBMC defined by CCR7⁺CD45RA⁺ (T_N; naïve), CCR7⁺CD45RA^- (T_CM; central memory), CCR7^-CD45RA^- (T_EM; effector memory), and CCR7^-CD45RA⁺ (T_EMRA; effector memory expressing CD45RA).